| Assay Method Information | |
| | Kinetic Studies |
| Description: | Inhibitor concentrations varied from 0.25 to 5 times the Ki value in 50 mM sodium phosphate, 100 mM sodium chloride buffer, pH 7.0, at 30 °C. 2-Chloro-4-nitrophenyl α-d-maltotrioside (CNP-G3) was used as the substrate in a similar range of concentrations. Initial reaction rates for the release of chloronitrophenolate were measured and the further progress of substrate cleavage monitored continuously. Reactions were performed on a Varian Cary 4000 UV/Vis spectrophotometer at 400 nm and 30 °C. |
| Affinity data for this assay | |
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