| Assay Method Information | |
| | DPP-IV Inhibition Activity |
| Description: | A 200-µL reaction system containing DPP-IV (Sigma), a test compound, and 25 mmol/L HEPES buffer (containing 140 mmol/L NaCl, 1% BSA, and 80 mmol/L MgCl2) was preincubated at room temperature for 10 min. The reaction was initiated by the addition of dipeptidyl peptidase DPPIV-GLOTM to the substrate H-Gly-Pro-AMC, and the reaction mixture was incubated at room temperature for 25-45 min. Thefluorescence intensity (F) at excitation 355 nm and emission 460 nm was then measured using photometer 5010 in 96-well plates. A negative control (no test compound) and a blank control (no enzyme) were run simultaneously. The tested compounds were initially assayed in duplicate at a concentration of 10 µg/mL |
| Affinity data for this assay | |
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