| Assay Method Information | |
| | Assay for α-Glucosidase Inhibitory Activity |
| Description: | The 135 µL of 50 mM phosphate saline buffer pH (6.8), solution of enzyme (20 µL), and 20 µL of test sample with 70% DMSO was added to 96-well plate. The samples were incubated for 15 min. Pre-read of the plate was taken after incubation using SpectraMax. substrate (pNPG) (25 µL) was added before final reading was takenat 400 nm on Spectra Max. |
| Affinity data for this assay | |
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