| Assay Method Information | |
| | GTPase Activity Assay |
| Description: | GTPase assays were performed in 50 mM MES, pH 6.5, 50 mM KCl, 5 mM MgCl2 in the presence of 2 uM FtsZ in the presence of varying concentrations of compound 1. Solutions were incubated at room temperature for 5 min, followed by addition of 0.25 mM GTP. At the end of a 20-min incubation period, enzyme activity was quenched by addition of 50 uL Malachite Green solution and the wells read at A625 on an absorbance plate reader. |
| Affinity data for this assay | |
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