| Assay Method Information | |
| | Cell-based Compound Screen |
| Description: | Compound stocks were prepared at a 10 mM concentration in dimethyl sulfoxide.T. brucei bloodstream forms (100 µl at 2.0 × 10^4 parasites/ml) were plated in 96-well plates and mixed with 100 µl of compounds diluted in HMI-9 medium with 10% FBS (Me2SO at <0.2%). Parasites not treated with compounds were also platedas controls. After 48 h of incubation at 37 °C and 5% CO2, 20µl of alamarBlue (Invitrogen) was added, and the assays were developed for 4 h. Fluorescence measurements were obtained using a SpectraMax M2 microplate reader (Molecular Devices) with excitation at 544 nm and emission at 590 nm (590-nm cutoff). |
| Affinity data for this assay | |
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