| Assay Method Information | |
| | Biochemical Assay (ATP at Km) |
| Description: | Briefly, MtPanK activity was measured in a pyruvate kinaselactate dehydrogenase-coupled assay where the reaction rate was determined by spectrophotometric measurement of NADH consumption. IC50 measurements were performed with a suitable concentration range of inhibitory compound, while keeping the concentration of pantothenate and ATP at 122 and 395 µM, respectively, which is their respective Km. |
| Affinity data for this assay | |
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