| Assay Method Information | |
| | In vitro Carbonic Anhydrase Inhibition Assay |
| Description: | CA activity was measured according to the method described by Verpoorte et al. in inhibition studies, spectrophotometrically [Biochem., 242:4221-4229]. The reaction mixture contains 50 mM Tris-SO4 buffer (pH = 7.4), 3 mM 4-nitrophenylacetate and enzyme solution in 1 ml total volume. A reference measurement was done by using the same cuvette without enzyme solution. The measurement was repeated in triplicate at each concentration of 5-amino-1,3,4-thiadiazole-2-sulfonamide containing pyrazole derivatives. |
| Affinity data for this assay | |
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