| Assay Method Information | |
| | MAO-A and MAO-B Fluorescence Assay |
| Description: | Recombinant human MAO-A and MAO-B were used for these studies and kynuramine served as enzyme substrate. Kynuramine is oxidised by both MAO isoforms to yield 4-hydroxyquinoline as ultimate product. For the enzyme reactions MAO-A (0.0075 mg/mL) or MAO-B (0.015 mg/mL), the substrate (50 μM) and the test inhibitors (0.003-100 μM) were combined and incubated for 20 min. For this purpose potassium phosphate buffer (pH 7.4, 100 mM) served as solvent. At endpoint the reactions were alkalinised (NaOH) and the fluorescence intensity of 4-hydroxyquinoline was measured (kex = 310 nm; kem = 400 nm). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |