| Assay Method Information | |
| | In Vitro α-Glucosidase Inhibitory Assay |
| Description: | The α-glucosidase inhibition assay had been carried out using baker's yeast α-glucosidase (EC 3.2.1.20) and p-nitrophenyl α-d-glucopyranoside. The test compounds were dissolved in DMSO to prepare the required distributing concentration. α-Glucosidase inhibitory activity was assayed by using 0.1 M phosphate buffer (pH 6.8) at 37 °C. The enzyme (0.1 U/mL) in phosphate buffer saline was incubated with various concentrations of test compounds at 37 °C for 15 min. Then 1.25 mM p-nitrophenyl α-d-glucopyranoside was added to the mixture as a substrate. After further incubation at 37 °C for 30 min. The absorbance was measured spectrophotometrically at 405 nm. The sample solution was replaced by DMSO as a control. Acarbose was used as a positive control. All experiments were carried out in triplicates. |
| Affinity data for this assay | |
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