| Assay Method Information | |
| | DHODH Biochemical Assay |
| Description: | Substrate-dependent inhibition of recombinant PfDHODH protein was assessed in an in vitro assay in 384-well clear plates (Corning 3702) as described by Malmquist et al. IC50 values were determined using a continuous assay where the reagent concentrations were 200 μM L-DHO, 18 μM CoQd, 100 μM dichloroindophenol (DCIP), and 2-10nM DHODH. |
| Affinity data for this assay | |
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