| Assay Method Information | |
| | Lipoxygenase Inhibition Assay |
| Description: | A mixture of test compound (10 μL; 1 mM) in MeOH, type-1B lipoxygenase (EC 1.13.11.12; from soyabean) (20 μL; 70 units) in 0.1 M aqueous phosphate buffer (pH 8.0) in a total volume of 160 μL was incubated for 10 min at 25°C. Then the reaction was initiated by the addition of a solution of linoleic acid as substrate (10 μL; 20 μM), resulting in the formation of (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate. The change in UV absorbance at 234 nm was followed over a period of 6 min. All reactions were performed in triplicate, and analyzed with a 96-well plate reader (SpectraMax Plus 384; Molecular Devices). |
| Affinity data for this assay | |
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