| Assay Method Information | |
| | In Vitro Assay |
| Description: | Human erythrocyte AChE or human plasmatic BChE (Prague, Aldrich; commercially purified by affinity chromatography) were suspended into phosphate buffer (pH 7.4) up to a final activity of 0.002 U/μL. Cholinesterase (5 μL), freshly mixed solution of 0.4 mg/mL 5,5'-dithio-bis(2-nitrobenzoic) acid (40 μL), 1 mM acetylthiocholine chloride in phosphate buffer (20 μL) and the appropriateconcentration of the inhibitor (1 mM-0.1 nM; 5 μL) were injected into each well. The absorbance was measured at 412 nm after 5 minutes incubation using automatedshaking of the microplate. All measurements were performedin triplicate. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |