| Assay Method Information | |
| | CA Activity Assay |
| Description: | CA activity was assayed in inhibition studies by following the change in absorbance at 348 nm of 4-nitrophenylacetate (NPA) to 4-nitrophenylate ion over a period of 3 min at 25°C using a spectrophotometer according to the method described by Verpoorte et al. [Verpoorte et al., J. Biol. Chem., 242:4221-4229] The enzymatic reaction, in a total volume of 1 mL, contained 50 mM Tris-SO4 buffer (pH = 7.4), 3 mM 4-nitrophenylacetate and enzyme solution. A reference measurement was done by using the same cuvette without enzyme solution. The inhibitory effects of some synthesized sulfonamide derivatives on CA enzyme activity purified from human erythrocyte were tested on three samples at each concentration used. |
| Affinity data for this assay | |
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