| Assay Method Information | |
| | Urease Inhibition Assay |
| Description: | Mixture of 25 μL of urease enzyme (Jack bean) solution containing 1 unit of enzyme (1 unit = 0.02 mg of enzyme) was dissolved in phosphate buffer of pH 6.8, 55 μL of buffer containing 100 mM urea, and 5 μL of test compounds (0.5 mM concentration) was incubated at 30°C for 15 min in 96-well plates. Urease activity was determined by measuring ammonia production using the iodophenol method38. Briefly, 45 μL each of phenol reagent and 70 μL of alkali reagent were added to each well. The increase in absorbance at 630 nm was measured after 50 min, using a microplate reader (Molecular Device, CA, USA). All reactions were performed in triplicate in a final volume of 200 μL. The results (change in absorbance per min) were processed using Soft-Max Pro software (Molecular Device, CA, USA). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |