| Assay Method Information | |
| | SARS-CoV PLpro Inhibition Assay |
| Description: | Reactions were performed in a total volume of 200 mL, which contained thefollowing components: 20mM Tris-buffer, pH 8.0, 10mM DTT, 30 mM Z-RLRGG-AMC, 2% DMSO and varying concentrations of inhibitor (0-200 mM). Assays were initiated with the addition of PLpro to produce a final enzyme concentration of 60 nM. |
| Affinity data for this assay | |
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