| Assay Method Information | |
| | Inhibition Activity Assay |
| Description: | ATX activity was determined by measurement of released choline in reactions containing ATX (10 nM), choline oxidase (0.1 U/ml), HRP (100 U/ml), amplex red (50 μM) and LPC 18:1 (10 μM). Compounds of the invention should be prepared as 10 point serial dilutions from 1 μM in duplicate and pre-incubated with ATX at 37° C. for 20 minutes prior to the addition of remaining reagents. The liberated choline was measured from changes in fluorescence intensity (λex 530 nm, λm 590 nm) of the product resurofin at 37° C. every 2 minutes over a 40-minute period. |
| Affinity data for this assay | |
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