| Assay Method Information | |
| | Evaluation of Inhibitory Activities Against GCS |
| Description: | Enzymatic reactions were initiated by sequentially adding the following reaction materials to a 96 deep-well plate. Thereafter, the enzymatic reactions were performed at 37° C. for 90 minutes. Enzyme reaction mix (Total 50 ul) Stock Con. Final Con. Volume (ul)1 Cpd. Mix Cpd. 500-0.003 uM 10000~0.056 nM 1 (DMSO) Total 12 Sub Mix 20 mM UDP-GIc 500 uM 1.25 25 mM EDTA 1 mM 2 500 uM NBD-Cer/BSA 5 uM 0.5 D.W 35.25 Total 393 Enzyme 3.135 ug/ul A549 0.627 ug/ul 10Mix cell lysate (31.35 ug) Total 10 |
| Affinity data for this assay | |
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