| Assay Method Information | |
| | Fluorescence Resonance Energy Transfer (FRET) Assay |
| Description: | FRET assay can be used to effectively monitor the inhibition of CBF binding to the Runt domain of RUNX proteins (as well as for CBFβ-SMMHC binding to the Runt domain of RUNX proteins). For the assay, the green fluorescent protein derivative Cerulean is fused to the N-terminus of the Runt domain and the green fluorescent protein derivative Venus to the N-terminus of CBFβ. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |