| Assay Method Information | |
| | Luminescent ATP/ADP Detection Assay |
| Description: | Briefly, the assay method measures ADP formed from EGFR kinase activity using luminescence derived from ATP-Luciferase detection system. Purified EGFR kinase was incubated with different concentrations of test compound in the kinase reaction buffer for 10 min. The reaction was stopped by depleting the unused ATP in the kinase reaction buffer using the depletion buffer for 40 min. The levels of ADP generated during the kinase reaction was detected by measuring the luminescence using the Luciferase system. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |