| Assay Method Information | |
| | Enzyme-Linked Immunosorbent Assay (ELISA) |
| Description: | EZH2Y641F: PRC2 complex experimental method: Experimental method: PRC2 complex (EZH2 Y641F/EED/SUZ12/RbAp48/AEBP2), H3(1-50) me1 substrate, methyl donor SAM and a compound were added into each well. The total reaction system was 104. Reaction was conducted in darkness at room temperature for 4 h. 5υ1 Eu-labeled H3K27 Me3 antibody and 5υ1 Streptavidin-XL665 were added into each well, mixed well and incubated for 1 h at room temperature, and fluorescence was measured at 620 nm and 665 nm with multi-label microplate assay system (PerkinElmer Envision), and the HTRF signal ratio per well (665 nm/620 nm) was calculated. The IC50 values of the compounds were calculated using SoftMax Pro 5.4.1 software. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |