Assay Method Information | |
| ChEMBL_2022253 (CHEMBL4676066) |
Description: | Inhibition of recombinant rat N-terminal His-tagged KHK expressed in Escherichia coli BL21 (DE3) using 8 mM fructose as substrate preincubated for 30 mins followed by 2 mM ATP addition and measured for 30 min by pyruvate kinase-lactate dehydrogenase coupled assay |
Affinity data for this assay | |
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