| Assay Method Information | |
| | LRRK2 Wild-Type ADP-Glo Protocol |
| Description: | In the assay, 0.5 nM LRRK2 WT kinase in kinase reaction buffer was incubated with the test compound (typically at 0 to 2 μM) for 30 minutes before the kinase reaction was initiated by addition of 1.0 mM ATP and 260 μM LRRKtide. The reaction mixture (10 μl total volume) was incubated for 3 hours at room temperature (RT, ca. 25° C.), before the reaction was terminated by addition of 10 μl ADP-Glo reagent. The mixture was further incubated for 60 minutes at RT before addition of 20 μl Kinase detection reagent from the ADP-Glo assay kit. Luminescence signal was measure on a BMG Labtech Pherastar FSX multimode microplate reader. The signal integration time was 0.5 s. |
| Affinity data for this assay | |
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