| Assay Method Information | |
| | Fluorescence-Based Assay |
| Description: | PSMA activity was also determined using a fluorescence-based assay according to a previously reported procedure. Briefly, lysates of LNCaP cell extracts were incubated with inhibitor in the presence of 4 μM NAAG. The amount of reduced glutamate was measured by incubating with a working solution of the Amplex Red glutamic acid kit (Molecular Probes Inc., Eugene, Oreg., USA). The fluorescence was determined by reading with a VICTOR3V multilabel plate reader (Perkin Elmer Inc., Waltham, Mass., USA) with excitation at 490 nm and emission at 642 nm. |
| Affinity data for this assay | |
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