| Assay Method Information | |
| | ATR Biochemical Potency Assay |
| Description: | ATR enzyme was made by ChemPartner (batch: CP-ATR-20161102-M2). 2× enzyme solutions were prepared in 1× kinase base buffer. 10 μl of 2× enzyme solution (final concentration: 2.5 nM) was added to each well of the 384-well assay plate containing 60 nl compound in each well. The plate was incubated at room temperature for 10 minutes. 2× peptide solutions were prepared with FAM-labeled peptide and ATP in the 1× kinase base buffer. 10 μl of 2× peptide solution was added to each well of the 384-well assay plate, which was incubated at 28° C. for 240 min. 40 μl of stop buffer was added to stop reaction. Data were collected by Caliper. |
| Affinity data for this assay | |
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