Assay Method Information

Assay Name:  BCL-xL TR-FRET Assay
Description:  BPS Bioscience, #50223: 10.5 ng of BCL-xL, 5 μL of 1:120 anti-His Tb-labeled donor, 5 μL of 1:120 Dye-labeled acceptor, 5 μL of 1:96 BCL-xL Peptide Ligand, 2 μL of 200× test compound, with 60 min incubation time (final concentration of DMSO 0.5%). The results of the assay were read using a plate reader with the following parameters: TR FRET, 340ex/620 and 665em; 60 usec Delay; and 500 usec integration.
Affinity data for this assay
 

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