| Assay Method Information | |
| | Isothermal Titration Calorimetry |
| Description: | Table 5: Experimental materials: the instrument for detecting heat change: ITC200 (Microcal, produced by GE Healthcare Company); buffer solution used by the dilution reagent: 50 mM of HEPES, 150 mM of NaCl, 0.5 mM of TCEP, and pH 7.5.Experimental method: All experiments were performed at 25° C. while the ITC buffer (50 mM of HEPES, 150 mM of NaCl, 0.5 mM of TCEP, and pH 7.5) was stirred at 1000 rpm. The titration of RORγ injection protein of all ligands was performed at an initial injection of 0.5 μL, followed by 20 identical 2 μL phase injections, with each injection lasting 4 seconds, at an injection interval of 180 seconds. The stock solutions of ligands and RORγ ligand protein were diluted with the ITC buffer to a concentration of 30 μM for the compounds and a concentration of 300 μM for the protein before titration. The final concentration of DMSO in the reaction buffer was less than 0.25% of the total volume. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |