| Assay Method Information | |
| | Inhibitory Activities of the Compounds on VEGFR2 |
| Description: | Detect compounds on VEGFR2 (KDR) by mobility shift assay. The initial test concentration of a compound was 1000 nM, 3 times dilution, 10 concentrations, and detection in duplicate. Nintedanib (Selleckchem, Cat. S1010) was used as the positive control compound. The operation method was briefly described as follows: kinase VEGFR2 (Carna, Cat. 08-191) at the final concentration of 0.5 nM and the test compound were mixed in the Optiplate-384F well plate and incubated for 10 minutes at room temperature. Then, ATP at a final concentration of 95 μM and 3 μM Kinase substrate 22 (Gill Biochemical (glbiochem), Cat. 112393) were added. After mixing, react at room temperature for 30 minutes. Add stop detection solution to terminate the reaction and read the conversion rate with Caliper EZ ReaderlI. |
| Affinity data for this assay | |
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