| Assay Method Information | |
| | MET Kinase ADP Glo Assay |
| Description: | Kinase assay was done by following instructions from the kit. Briefly, a ten point serial dilution of compound was prepared at 5× in assay buffer with the final assay concentrations starting at 3000 nM, 1000 nM, 300 nM, 100 nM . . . 0 nM. Enzyme, substrate and ATP were used at 25 ng, 2000 ng, and 25 M, respectively. The assay plate was set up by mixing the components in a total reaction volume of 10 μL per well. The plate was centrifuged gently for 10 seconds and incubated at room temperature for 60 minutes in the dark. The ADP-Glo Reagent and kinase detection reagent were added and incubated as recommended. The reaction was quantified by measuring luminescence on the Perkin Elmer Envision plate reader. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |