| Assay Method Information | |
| | Measurement of Dissociation Constant |
| Description: | Dissociation constant of 667-coumate was measured for tight-binding inhibitors of CA II. The formation of the 4-nitrophenol was monitored at 348 nm for 2 min using a PerkinElmer Lambda 4.0 spectrometer, and rates were derived using Kinlab software. Inhibition constants were determined with 667-coumate at final concentrations of 33, 83, 166 and 250 nM, with each assay conducted at least in triplicate. |
| Affinity data for this assay | |
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