Assay Method Information

Assay Name:  alpha-Adrenoceptor Radioligand Binding Assay
Description:  The binding affinity of compound was evaluated using [3H] Clonidine as ligand in competition binding experiments. Nonspecific binding was determined as the concentration of ligand bound in the presence of 2 uM phentolamine. Ki values were determined by the equation: Ki = IC50/(1 + [clonidine]/KD).
Affinity data for this assay
 

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