| Assay Method Information | |
| | CDK1 Enzymatic Assay |
| Description: | Human CDK1 kinase activity was assayed in reaction buffer containing substrate Histone H1, enzyme, and inhibitor in the presence of 100uM ATP/[gamma-33P] ATP. 33P incorporation was measured using a Perkin-Elmer Trilux scintillation counter. The IC50 is the inhibitor concentration, which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from 33P labeled ATP to the substrate. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |