Assay Method Information

Assay Name:  ChEMBL_1852989 (CHEMBL4353613)
Description:  Inhibition of human N-terminal His-tagged SIRT2 expressed in Escherichia coli using p53 (Gln-Pro-Lys-Lys(Ac)) (317 to 320 residues) as substrate incubated for 3 hrs measured up to 30 mins in presence of NAD+ by fluorescence-based Michaelis-Menten plot analysis
Affinity data for this assay
 

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