| Assay Method Information | |
| | ChEMBL_2315916 |
| Description: | Inhibition of 6xHis-tagged human DNA polymerase eta (1 to 437 residues) expressed in Escherichia coli BL21 DE3 assessed as displacement of reporter strand using 5'-TTT TTT TTG C-TAMRA-3' reporter/5'-TCA CCC TCG TAC GAC TCT T-3' primer/5'-BHQ2-GCA AAA AAA AAA GAG TCG TAC GAG GGT GA-3' template of 1:1.5:1.5 as DNA substrate preincubated for 5 to 10 mins followed by substrate addition measured immediately in presence of dTTP/MgCl2 by fluorescence based assay |
| Affinity data for this assay | |
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