Assay Method Information | |
| ChEMBL_1454388 (CHEMBL3364860) |
Description: | Inhibition of hexa-His-tagged recombinant human SIRT1 expressed in Escherichia coli BL21(DE3) using AMC-labeled Arg-His-Lys-Lys(ac) as substrate assessed as inhibition of deacetylation of peptide after 45 mins by Lineweaver-Burke plot method |
Affinity data for this assay | |
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