| Assay Method Information | |
| | ChEMBL_1587139 (CHEMBL3825739) |
| Description: | Inhibition of human recombinant GST-tagged PAK4 kinase domain (295 to 591 residues) expressed in baculovirus expression system assessed as suppression of phosphorylation of coumarin/fluorescein-labelled FRET peptide substrate at Ser/Thr19 residues preincubated with substrate for 10 mins followed by enzyme addition measured after 60 mins by Z-Lyte assay |
| Affinity data for this assay | |
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