| Assay Method Information | |
| | Lipo-oxygenase activity assay |
| Description: | The assay was progressed in 96-well microliter plates containing substrate, soybean LOX enzyme, and respective test substance at room temperature in a 0.1 M Tris-HCl buffer solution at pH 7. The reaction was initiated by the addition of substrate solution to all wells and incubated for 5 min, followed by the addition of 100 µL of chromogen solution to all wells to stop enzyme catalysis and incubated for 5 min. The absorbance was measured at 490 nm. Dimethyl sulphoxide served as a negative control. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |