Assay Method Information

Assay Name:  GW0385 Assay
Description:  The assay method employed kinetic determinations of values for k1 and k-1, from which value of inhibition constant (Ki ) was determined (k-1/k1). The k1 is calculated from spectrofluorometric data in the presence of saquinavir. The k-1 is calculated from the time course for displacement of [3H]GW0385 from E[3H]GW0385 by nonlabeled GW0385.
Affinity data for this assay
 

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