| Assay Method Information | |
| | JAK3 Enzymatic Assay |
| Description: | The assay has been performed using the JAK3 kinase domain (residues 781-1124 of the 1124 amino acid long full-length sequence, accession number P52333 of UniProtKB/Swiss-Prot databse). The JAK3 kinase domain showed a linear kinetic without prephosphorylation. The JAK3 kinase assay was run with a final enzyme concentration of 1 nM, in the presence of 22 uM ATP, 1 nM 33P-γ-ATP and 40 uM of substrate BioDBn*306 (Amino acid sequence: LPLDKDYYWREPGQ-SEQ ID NO: 1). The peptidic substrate was purchased from American Peptide Company (Sunnyvale, Calif.). |
| Affinity data for this assay | |
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