| Assay Method Information | |
| | Fluorometric Activity Assay |
| Description: | A FLUOR DE LYS fluorometric activity assay kit (HDAC source: HeLa cell nuclear extract) and a FLUOR DE LYS HDAC1 fluorometric drug discovery assay kit (HDAC source: human recombinant HDAC1) were purchased from Enzo Life Sciences (Farmingdale, NY). HDAC inhibition assay was performed according to the instruction manuals. All samples were dissolved in dimethyl sulfoxide, except for 1 and 2, which were dissolved in the assay buffers provided. All assays were performed in triplicate, and errors were calculated as standard deviation. |
| Affinity data for this assay | |
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