| Assay Method Information | |
| | Inhibition Assay |
| Description: | Inhibitory activities of compounds against KDR (VEGFR2) were also measured by Invitrogen using Z'-LYTE Method as described above with the following modification. The 2x KDR (VEGFR2)/Tyr 01 mixture is prepared in 50 mM HEPES pH 7.5, 0.01% B RIJ-35, 10 mM MgCl2, 1 mM EGTA. The final 10 μL Kinase Reaction consists of 0.5-11.7 ng KDR (VEGFR2) and 2 μM Tyr 01 in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. After the 1 hour Kinase Reaction incubation, 5 μL of a 1:256 dilution of Development Reagent B is added. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |