| Assay Method Information | |
| | Competition Radioligand Binding Assay |
| Description: | A2A Adenosine Receptor: Competitive assays were carried out by incubation of membranes from human A2A receptors transfected to HeLa cells, [3H]-ZM241385, buffer (Tris-HCl 50 mM (pH=7.4), 10 mM MgCl2, EDTA 1 mM and 2 units/ml adenosine deaminase), and unlabelled ligand in a total volume of 0.2 ml for 60 min at 25° C. NECA was used to determine nonspecific binding. It was filtered over Schleicher & Schuell GF/52 (presoaked with 0.5% polyethyleneimine) in a Brandel cell harvester. The unbound radioligand was removed with 3×250 μl of 20 mM Tris-HCl 50 mM (pH=7.4), 10 mM MgCl2, EDTA 1 mM. |
| Affinity data for this assay | |
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