| Assay Method Information | |
| | Inhibition of CSF-1 Induced Monocyte MCP-1 Expression Cell Based Assay |
| Description: | Representative compounds of the present invention were additionally tested in an in vitro assay measuring inhibition of CSF-1 induced monocyte MCP-1 expression, as described briefly below.Human monocytes were isolated from the blood of three donors by negative selection using RosetteSep human monocyte enrichment cocktail from StemCell Technologies (Cat. #15068). Monocytes (1×105/well) were cultured in 96 well polypropylene plates (Corning 3790) with Roswell Park Memorial Institute (RPMI) 1640 media containing 10% heat-inactivated fetal bovine serum and 1% Penicillin-Streptomycin, Liquid (complete media). Cultures were adjusted to contain graded concentrations (0.33 to 0.000151 micromolar or zero micromolar) of test compound and 10 ng/mL human CSF-1. Conditioned media were harvest after approximately eighteen hours of culture and assayed for MCP-1 protein using a specific ELISA. |
| Affinity data for this assay | |
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