| Assay Method Information | |
| | ChEMBL_1855278 (CHEMBL4356007) |
| Description: | Inhibition of N-terminal His6-tagged Zika virus NS2B (45 to 96 residues) - NS3 (1 to 177 residues) protease domain expressed in Escherichia coli BL21-Gold (DE3) cells or Escherichia coli BL21 Rosetta (DE3) preincubated for 30 mins followed by Bz-nKRR-AM substrate addition measured over 10 mins at 60 secs interval by fluorescence method |
| Affinity data for this assay | |
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