| Assay Method Information | |
| | Enzyme Assay |
| Description: | JAK1: The 2×JAK/Tyr 06 (ThermoFisher Scientific proprietary) mixture was prepared in 50 mM HEPES pH 6.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA, 0.02% NaN3. The final 10 μL Kinase Reaction consisted of 74 nM JAK, 2 μM Tyr 06 and 75 μM ATP (Km app measured as 87 μM ATP) in 50 mM HEPES pH 7.0, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA, 0.01% NaN3. After the 1 hour Kinase Reaction incubation, 5 μL of a 1:128 dilution of Development Reagent was added. |
| Affinity data for this assay | |
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