| Assay Method Information | |
| | PKA Enzymatic Assay |
| Description: | Human PKA kinase activity was assayed in reaction buffer containing substrate Kemptide, enzyme, and inhibitor in the presence of 100uM ATP/[gamma-33P] ATP. 33P incorporation was measured using a Perkin-Elmer Trilux scintillation counter. The IC50 is the inhibitor concentration, which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from 33P labeled ATP to the substrate. |
| Affinity data for this assay | |
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