| Assay Method Information | |
| | ChEMBL_1907069 (CHEMBL4409427) |
| Description: | Inhibition of wild type recombinant human C-terminal His8-tagged IDH1 expressed in Escherichia coli BL21 assessed as reduction in NADP consumption using DL-isocitrate as substrate preincubated for 60 mins followed by substrate addition and measured for 60 mins in presence of NADP by diaphorase/resazurin-based fluorescence assay |
| Affinity data for this assay | |
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