Assay Method Information

Assay Name:  ChEMBL_1454386 (CHEMBL3364858)
Description:  Inhibition of human SIRT1 I347A mutant expressed in Escherichia coli BL21(DE3) using AMC-labeled Arg-His-Lys-Lys(ac) as substrate assessed as inhibition of deacetylation of peptide after 45 mins by Lineweaver-Burke plot method
Affinity data for this assay
 

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