| Assay Method Information | |
| | Radioligand Binding Assay, 5HT6 |
| Description: | Materials and Methods:Receptor Source: Human recombinant 5-HT6 expressed mammalian cellsRadioligand: [125I] SB258585, 15 nM or [3H]LSD, 2 nMControl Compound: Methiothepin or serotoninIncubation Conditions: The reactions were carried out in 50 mM TRIS-HCl (pH 7.4) containing 10 mM MgSO4, 0.5 mM EDTA and 0.1% Ascorbic acid at room temperature for 1 hour. The reaction was terminated by rapid vacuum filtration onto glass fiber filters. Radioactivity trapped onto the filters was determined and compared to control values in order to ascertain any interactions of test compounds with the cloned serotonin-5HT6 binding site. |
| Affinity data for this assay | |
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