| Assay Method Information | |
| | Radioligand Binding Assay, 5HT7 |
| Description: | Materials and Methods: Receptor Source: Human recombinant 5-HT7 expressed CHO cells Radioligand: [3H] Lysergic acid diethylamide (LSD), 4 nM Control Compound: Serotonin Incubation Conditions: The reactions were carried out in 50 mM TRIS-HCl (pH 7.6) at room temperature for 90 minutes. The reaction was terminated by rapid vacuum filtration onto glass fiber filters. Radioactivity trapped onto the filters was determined and compared to control values in order to ascertain any interactions of test compounds with the serotonin-5HT7 binding site. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |