| Assay Method Information | |
| | ChEMBL_2031799 (CHEMBL4685957) |
| Description: | Inhibition of human N-terminal His-tagged SIRT3 (122 to 391 residues) expressed in Escherichia coli using Ac-Arg-Leu-Ile-Lys(Ac)-AMC as substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins in presence of NAD+ by fluorescence assay |
| Affinity data for this assay | |
|---|---|
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